Rapid Lentivirus Titration Cassette ( LentiTitre) is a lentivirus titration kit for rapid and accurate estimation of the functional lentivirus titer in less than 10 minutes. LentiTitre is a lateral flow immunoassay (LFIA) based titration device, based on p24 detection with sensitive gold-conjugated antibody. Lentiviral supernatant standards functionally titrated at 106 TU/mL are included in the kit. By comparing the intensities of the test lines of the standards and that of unknown sample, the cassette can help accurately estimate lentiviral TU titer, using p24 as a gauge. It can also be used to determine the optimal time when packaging preparation is ready for harvesting. 

Features 

• Simple and quick: Load virus sample and read titer in 10 minutes.
• 独特和安全：提供预滴定的慢病毒标准，但灭活以确保安全。 
• 对大范围敏感：  检测低至10 ^ 4 TU / ml和高达10 ^ 6 TU / ml的慢病毒滴度。
• 灵活的选项：多个样品可以与同一组标准一起滴定。

协议

• 准备标准：直接从提供的样品瓶中使用10 ^ 6 TU / ml原液。通过用细胞培养基稀释至所需的滴度来制备另外两种标准品。建议10倍稀释至10 ^ 5TU / ml和10 ^ 4TU / ml，或5倍稀释至2×10 ^ 5TU / ml和4×10 ^ 4TU / ml。
• 水平放置磁带。
• 将100μL的每种标准品加入其中一个样品孔中。
• 将100μL包装上清液加入其中一个样品孔中。
• 等待10分钟
• 控制线将始终显示。通过比较其测试线的强度与标准品的强度来确定未知慢病毒制剂的滴度。