Lentiviral vector-tet-inducible, Puro contains  Multiple Cloning Sites (MCS) downstream of the tet inducible promoter, and puromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by puromycin.

Extremely low background without induction, high level of protein expression after induction with doxycycline in the present of rtTA
Extremely Low recombination rate
Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System  
  
Vector:    pLV-tet-MCS-PGK-Puro
DNA amount    10 µg
Vector Information:    Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene:    tet inducible element
Selection Gene:    Puromycin
Promoter for Selection Gene    PGK
Biosafety Level:    BSL-2
Shipped:    Room Temperature
Storage:    Store at -20°C
Plasmid Map