Lentiviral vector-EF1a, Puro contains  Multiple Cloning Sites (MCS) downstream of the EF1 alpha promoter, and puromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as 293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by puromycin.

High Protein Expression Levels
Extremely Low recombination rate
Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System  
  
Vector:    pLV-EF1a-PGK-Puro
DNA amount    10 µg
Vector Information:    Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene:    EF1a
Selection Gene:    Puromycin
Promoter for Selection Gene    PGK
Biosafety Level:    BSL-2
Shipped:    Room Temperature
Storage:    Store at -20°C

Plasmid Map